EGFR Mouse Monoclonal Antibody

• Catalog Number: orb500624
• Category: Antibodies
• Description: EGFR Mouse Monoclonal Antibody
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR
• UniProt ID: P00533
• MW: 130 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Antibody Type: Primary Antibody
• Clone Number: 13B2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR; Avian erythroblastic leukemia viral(v erb b)
• Research Area: Cancer Research, Cell Biology, Cell Differentiatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-EGFR (orb500624) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 170 kD, Observed band size: 180 kD.

Sample: Hela KO EGFR (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-EGFR (orb500624) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 170 kD.