EGFR mutant Antibody

SKU: orb153451

Description

Mouse monoclonal antibody to EGFR mutant (L858R)

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 5G9B5
• Immunogen: Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
• Target: EGFR mutant
• Molecular Weight: 175kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

ERBB; HER1; mENA; ERBB1; PIG61

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR mutant antibody

Flow cytometric analysis of HepG2 cells lysates using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using EGFR mutant antibody

Western blot analysis of human EGFR mutant using EGFR mutant antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR mutant antibody

Western blot analysis of SPC-A-1 (Lane 1), A549 (Lane 2), HepG2 (Lane 3) MCF-7 (Lane 4) cell lysate using EGFR mutant antibody

Quick Database Links

Gene Symbol

EGFR mutant

UniProt

P00533

NCBI Gene ID

1956