EGFR mutant Antibody

• Catalog Number: orb153451
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR mutant (L858R)
• Target: EGFR mutant
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Immunogen: Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
• UniProt ID: P00533
• MW: 175kDa
• Tested applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Antibody Type: Primary Antibody
• Clone Number: 5G9B5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; HER1; mENA; ERBB1; PIG61
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR mutant antibody

Flow cytometric analysis of HepG2 cells lysates using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using EGFR mutant antibody

Western blot analysis of human EGFR mutant using EGFR mutant antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR mutant antibody

Western blot analysis of SPC-A-1 (Lane 1), A549 (Lane 2), HepG2 (Lane 3) MCF-7 (Lane 4) cell lysate using EGFR mutant antibody