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Biorbyt provides end-to-end protein immunology detection services engineered to support target validation, mechanistic research, and analytical profiling. Our platform integrates optimized sample preparation with high-sensitivity immunodetection to characterize protein expression levels, protein-protein interactions, and protein-nucleic acid binding complexes.
From total protein profiling and immunoprecipitation to electrophoretic mobility shift assays (EMSA) and quantitative optical densitometry, our standardized workflows ensure high reproducibility, minimal background noise, and publication-ready report delivery.
Core Detection Services
Western Blot (WB)
High-resolution immunoblotting workflows for target protein identification, post-translational modification analysis, and expression profiling.
- Protein Extraction & Quantification (BCA / Bradford)
- SDS-PAGE Gel Electrophoresis
- High-Sensitivity Chemiluminescent (ECL) Imaging
Protein Interaction Assays
Targeted affinity capture techniques to isolate protein complexes, confirm physical interactions, and map intracellular pathways.
- Immunoprecipitation (IP) of endogenous targets
- GST / His Tag Pull-Down Assays
- Co-Immunoprecipitation (Co-IP) for intact complexes
Coomassie Blue Staining
Colloidal staining assays for total protein visualization, purity assessment, and downstream mass spectrometry compatibility.
- Coomassie Brilliant Blue Gel Staining
- Destaining & High-Resolution Band Visualization
- Total Protein Profiling & Purity Verification
EMSA (Shift Assays)
Gel mobility shift assays to investigate transcriptional regulation, transcription factor binding, and nucleoprotein complex affinity.
- Electrophoretic Mobility Shift Assay (EMSA)
- Protein-DNA / Protein-RNA Interaction Analysis
- Probe Labeling (Biotin) & Competition Assays
Data Analysis & Report
Rigorous quantitative analysis ensuring experimental datasets are fully prepared for publication or regulatory filing.
- Optical Densitometry Band Quantification
- Normalization to Housekeeping Loading Controls
- Statistical Graphing & Full Report Delivery