EGFR Antibody

• Catalog Number: orb153505
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7A6F12
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 175kDa
• Target: EGFR
• Entrez: 1956
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: ERBB; HER1; mENA; ERBB1; PIG61
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR antibody

Flow cytometric analysis of A431 cells lysates using EGFR antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded endometrial cancer tissues using EGFR antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using EGFR antibody

Western blot analysis of human EGFR using EGFR antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR antibody

Western blot analysis of A431 (Lane 1) Hela (Lane 2) cell lysate using EGFR antibody