Antibody Conjugation

Antibody conjugation significantly enhances experimental sensitivity, specificity, and assay versatility. Fluorescently labeled antibodies enable direct visualization in flow cytometry and immunofluorescence microscopy, while biotinylated targets streamline detection across high-throughput ELISA and Western blot platforms.

Benefits of Custom Antibody Conjugation

Enhanced Assay Sensitivity & Specificity

Direct antibody conjugation eliminates secondary antibody cross-reactivity and background noise, boosting signal-to-noise ratios across complex immunofluorescence and flow cytometry panels.

Fast Laboratory Turnaround

Conjugations are performed on a made-to-order basis, taking just 1–2 weeks in addition to the base antibody lead time.

Custom Antibody Conjugation Services

Biorbyt provides custom conjugation options across a wide variety of catalog and customer-supplied antibodies. Prices are structured per 100 µg of antibody, with options available for biotinylation as well as bright, stable fluorophores. Please enquire with our technical team prior to ordering to verify antibody formulation and buffer compatibility.

Conjugation	Abs/Em Maxima	Excitation Laser	Dye Profile	Application Details
Biotin	N/A	N/A	N/A	Ideal for streptavidin amplification in ELISA and Western blotting.
FITC	494 / 518 nm	488 nm	Green	Classic choice for flow cytometry and immunofluorescence microscopy.
CF 405M	408 / 452 nm	405 nm	Violet	Optimized for violet laser lines in multicolor flow cytometry panels.
CF 488A	490 / 515 nm	488 nm	Green	Significantly brighter and more photostable alternative to FITC.
CF 594	593 / 614 nm	561 to 568 nm	Deep Red	Excellent brightness for multiplexed flow cytometry and imaging.
CF 647	650 / 665 nm	633 / 635 / 640 nm	Red	Far-red dye minimizing autofluorescence in complex tissue samples.

Pricing, buffer requirements, and technical details are available via our Custom Conjugation Page or by contacting our team at .