EGFR Mouse Monoclonal Antibody (Cy5)

• Catalog Number: orb969172
• Category: Antibodies
• Description: EGFR Mouse Monoclonal Antibody (Cy5)
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Cy5
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 13B2
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: EGFR; Avian erythroblastic leukemia viral(v erb b)
• Research Area: Cancer Research, Cell Biology, Cell Differentiatio
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-EGFR Antibody, conjugated 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.