Recombinant Protein Expression

 

Recombinant protein expression involves the use of genetic engineering techniques to introduce a gene encoding a desired protein into a host organism, such as bacteria, yeast, insect cells, or mammalian cells, which then produce the protein. This process typically includes cloning the gene into an expression vector, which contains regulatory elements to drive protein production, followed by transformation or transfection of the vector into the host organism. The host organism then synthesizes the recombinant protein according to the instructions encoded in the introduced gene, allowing for the large-scale production of specific proteins for various purposes, including research, therapeutic, and industrial applications.

Why Choose Biorbyt

• Competitive pricing
• 39 different tags to pick from
• High purity >85%
• Capacity for achieving large-scale protein production (10 mg, 50 mg, 100 mg, 200 mg quantities available)
• Complimentary additional services offered: Desalination, aliquoting, endotoxin elimination, aseptic processing and lyophilization

Expression systems

In vitro E.coli Expression System

• High yield - some can reach as high as 5mg/ml
• High-throughout
• Fewer steps and a simple experimental process
• The open reaction system allows for easy adjustment of reaction conditions, aiding in the regulation of gene transcription, protein synthesis, and post-translational modification.

E.coli Expression System

• Clear genetic background
• Cost-effective
• Short lead time
• Fast growth and high yield
• High success rate
• Easy to optimise to achieve the best results

Yeast Expression System

• High expression level
• No self-produced endotoxin
• Can be properly folded
• More stable than prokaryotic proteins
• Products undergo post-translational modifications such as glycosylation, phosphorylation, and acylation, increasing the likelihood of possessing biological activity

Insect Baculovirus Expression System

• Large capacity: ability to carry large gene fragment; advantage in large protein expression
• Strict species specificity
• High expression efficiency
• Amplifying Baculovirus is simpler, and it's capable of producing recombinant proteins on a large scale.

Mammalian Cell Expression System

• No-self produced endotoxin
• Featuring an array of intricate N-linked glycosylation, precise O-linked glycosylation, and additional post-translational processing
• Close to native protein in the molecular structure
• High yield
• Multi-cell lines
• Utilizing various cell lines and multiple expression techniques boosts both the success rate and yield of protein expression

 

Select Expression SystemE.coliin vitro E.coliYeastInsect cellsMammalian cells

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