EGFR Antibody (C-term)

SKU: orb1939233

Description

EGFR Antibody (C-term)

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:8000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Molecular Weight: 134277 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

ERBB, ERBB1, HER1

All lanes: Anti-EGFR Antibody (C-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EGFR Antibody (C-term) at 1:8000 dilution. Lane 1: A549 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: MDA-MB-231 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Quick Database Links

UniProt

P00533