EGFR Mouse Monoclonal Antibody (FITC)

• Catalog Number: orb463741
• Category: Antibodies
• Description: EGFR Mouse Monoclonal Antibody (FITC)
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: FITC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 13B2
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: EGFR; Avian erythroblastic leukemia viral(v erb b)
• Research Area: Cancer Research, Cell Biology, Cell Differentiatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A549. Primary Antibody (green line): Mouse Anti-EGFR antibody (orb463741), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-EGFR antibody (orb463741), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-EGFR Antibody, conjugated (orb463741 EGFR) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.