Sonic Hedgehog Antibody

SKU: orb1338678

Description

Mouse monoclonal antibody to Sonic Hedgehog (SHH)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:2000, IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: BQVK5C4
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
• Target: Sonic Hedgehog
• Molecular Weight: 27.6 kDa (Actual 22 kDa/55 kDa)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed using Sonic Hedgehog (SHH) antibody

Quick Database Links

Gene Symbol

Sonic Hedgehog

UniProt

Q15465

RefSeq (mRNA):NM_000193