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Catalog Number | orb1926670 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB51214 |
Tested applications | FC, WB |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, FC: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 47773 Da |
Target | This Mouse Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of mouse Shh. |
UniProt ID | Q62226 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Sonic hedgehog protein, SHH, HHG-1, Sonic hedgehog Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of Hela cells using (Mouse) Shh Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from mouse liver tissue lysate, using Shh Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.