Shh Rabbit Polyclonal Antibody

• Catalog Number: orb11368
• Category: Antibodies
• Description: Shh Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Shh (21-120/462aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 19/47 kDa
• Target: SHH
• UniProt ID: Q15465
• RRID: AB_10747540
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Sonic hedgehog; Vhh-1; Sonic hedgehog protein; SHH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fan, Haixia et al. Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts J Exp Clin Cancer Res, 36, 163 (2017)

PubMed: 29157266

Paraformaldehyde-fixed, paraffin embedded (Mouse stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (orb11368) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (orb11368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Embryo (Mouse) tissue at 30 ug, Primary: Anti-Shh (orb11368) at 1:300 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 19/47kD, Observed band size: 50kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Shh (orb11368) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19/47kD, Observed band size: 49 kD.