SHH Antibody

• Catalog Number: orb527297
• Category: Antibodies
• Description: Goat polyclonal antibody to SHH
• Target: SHH
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DSEALHPLGMAVK
• MW: 49.6
• Tested applications: ELISA, FC, IF, WB
• Dilution range: P-ELISA: 1: 32000
• Application notes: WB: Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular . Notes: Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SHH, sonic hedgehog homolog (Drosophila), HGNC:108
• Note: For research use only
• Entrez: 6469
• Expiration Date: 12 months from date of receipt.

WB analysis of Human Liver lysate using SHH antibody

Immunofluorescence analysis of HeLa cells using SHH antibody

Immunofluorescence analysis of NIH3T3 cells using SHH antibody

Flow cytometric analysis of HeLa cells using FOXC2 antibody

Immunofluorescence analysis of U251 cells using SHH antibody