SIAH1 Antibody

SKU: orb18357

Description

Goat polyclonal antibody to SIAH1

Research Area

Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 37kDa band observed in Human Liver lysates and approx. 30+37kDa bands in Rat Liver lysates (calculated MW of 34.6kDa according to Human NP_001006611.1 and 31.1kDa according to Rat NP_543181.2 and 38.2kDa according to Rat XP_038953364.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10µg/ml.
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SIAH1
• Protein Sequence: SRQTATALPTGTSKC
• Molecular Weight: 31.1; 34.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SIAH1 antibody, anti seven in absentia homolog 1 (Drosophila) antibody, anti Siah-1 antibody, anti hSIAH1 antibody, anti HUMSIAH antibody, anti Siah-1a antibody, anti FLJ08065 antibody, anti seven in absentia homolog 1 antibody, anti sonic hedgehog homolog antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody.

Primary incubation 1 hour at room temperature. Images A+B: Human Liver lysate + peptide, incubation at primary Ab concentration 1 ug/ml, C+D: Rat Liver lysate + peptide, incubation at primary Ab concentration 3 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SIAH1

NCBI Gene ID

6477