Cart summary

You have no items in your shopping cart.

SIAH1 Antibody

SKU: orb18357

Description

Goat polyclonal antibody to SIAH1

Research Area

Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsELISA, IF, WB
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 37kDa band observed in Human Liver lysates and approx. 30+37kDa bands in Rat Liver lysates (calculated MW of 34.6kDa according to Human NP_001006611.1 and 31.1kDa according to Rat NP_543181.2 and 38.2kDa according to Rat XP_038953364.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10µg/ml.
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Mouse

Key Properties

HostGoat
ClonalityPolyclonal
TargetSIAH1
Protein SequenceSRQTATALPTGTSKC
Molecular Weight31.1; 34.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti SIAH1 antibody, anti seven in absentia homolog 1 (Drosophila) antibody, anti Siah-1 antibody, anti hSIAH1 antibody, anti HUMSIAH antibody, anti Siah-1a antibody, anti FLJ08065 antibody, anti seven in absentia homolog 1 antibody, anti sonic hedgehog homolog antibody

Similar Products

  • Rabbit Human SIAH1, E3 Ubiquitin Ligase Antibody [orb109020]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Rabbit Human SIAH1, E3 Ubiquitin Ligase Antibody [orb198345]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg
  • SIAH1 Antibody [orb1258154]

    IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • SIAH1 Rabbit Polyclonal Antibody [orb329760]

    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • SIAH1 Rabbit Polyclonal Antibody [orb576575]

    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

SIAH1 Antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody

SIAH1 Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody.

SIAH1 Antibody

Primary incubation 1 hour at room temperature. Images A+B: Human Liver lysate + peptide, incubation at primary Ab concentration 1 ug/ml, C+D: Rat Liver lysate + peptide, incubation at primary Ab concentration 3 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SIAH1 Antibody (orb18357)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Available Sizes

Select a size below

100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
Bulk Enquiry