LGR5/GPR49 Antibody

• Catalog Number: orb1939305
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Target: This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5/GPR49.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: O75473
• MW: 99998 Da
• Tested applications: FC, WB
• Dilution range: WB: 1：2000, FC: 1:25
• Antibody Type: Primary Antibody
• Clone Number: 349CT16.1.3.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leucine-rich repeat-containing G-protein coupled r
• Note: For research use only
• NCBI: NP_001264155.1, NP_003658.1

Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western blot analysis of lysates from K562 cell line (from left to right), using LGR5/GPR49 Antibody (Center). diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 20 μg per lane.