LGR5 Antibody

• Catalog Number: orb1264632
• Category: Antibodies
• Description: LGR5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: LGR5
• UniProt ID: O75473
• NCBI: O75473
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Leucine-rich repeat-containing G-protein coupled r
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of in K562 cell line lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow) (Kindly offered by Dr. Li).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.