LGR5/GPR49 Antibody

• Catalog Number: orb1264634
• Category: Antibodies
• Description: LGR5/GPR49 Antibody
• Target: LGR5
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Immunogen: This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.
• UniProt ID: O75473
• MW: 100 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Leucine-rich repeat-containing G-protein coupled r
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only
• NCBI: O75473

Western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of LGR5/GPR49 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).