LGR5 (GPR49) Antibody (Center)

• Catalog Number: orb1931495
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: O75473
• MW: 99998 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC: 1:100, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB14555
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leucine-rich repeat-containing G-protein coupled r
• Note: For research use only
• NCBI: NP_003658.1, NP_001264155.1

LGR5 (GPR49) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. Diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-LGR5/GPR49 Antibody (Center) in K562 cell line lysates (35 ug/lane). LGR5/GPR49 (arrow) was detected using the purified Pab.

LGR5/GPR49 Antibody (Center) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).