LGR5 (GPR49) Antibody (Center)

SKU: orb1931495

Description

LGR5 (GPR49) Antibody (Center)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IHC - 1:100
• Reactivity: Human
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 99998 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

GPR49, GPR67

LGR5 (GPR49) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. Diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-LGR5/GPR49 Antibody (Center) in K562 cell line lysates (35 ug/lane). LGR5/GPR49 (arrow) was detected using the purified Pab.

LGR5/GPR49 Antibody (Center) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Quick Database Links

UniProt

O75473