LGR5 antibody (N-term)

SKU: orb1925279

Description

LGR5 antibody (N-term)

Research Area

Cancer Biology, Pharmacology & Drug Discovery, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution range: WB - 1:1000
• Reactivity: Hamster, Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 99998 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

GPR49, GPR67

All lanes: Anti-LGR5 antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Observed band size: 100 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of LGR5 Rabbit Brain tissue was performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (Ready-to-use) for 15 min at room temperature.

Immunohistochemical analysis of LGR5 Rabbit Small intestine tissue was performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (Ready-to-use) for 15 min at room temperature.

Quick Database Links

UniProt

O75473