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LEF1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | lymphoid enhancer-binding factor 1 |
| Target | LEF1 |
| Molecular Weight | 40-45 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
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COLO 320 cells were subjected to SDS PAGE followed by western blot with orb628412 (LEF1 Antibody) at dilution of 1:300.

IP Result of anti-LEF1 (IP: orb628412, 4 ug; Detection: orb628412 1:300) with SW 1990 cells lysate 2400 ug.

Immunohistochemistry of paraffin-embedded human thymus using orb628412 (LEF1 antibody) at dilution of 1:100.
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Protocol Information
LEF1 Antibody (orb628412)
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