LEF-1 Rabbit Polyclonal Antibody

• Catalog Number: orb13540
• Category: Antibodies
• Description: LEF-1 Rabbit Polyclonal Antibody
• Target: LEF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human LEF-1 (331-399/399aa)
• UniProt ID: Q9UJU2
• RRID: AB_10753437
• MW: 44 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: lymphoid enhancing factor-1; Transcription factor
• Note: For research use only

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-LEF-1 antibody (orb13540), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Spleen tissue lysates, Primary: Anti-LEF-1 (orb13540) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 55 kDa.

Sample: Lymph nodes (Mouse) Lysate at 30 ug, Primary: Anti-LEF-1 (orb13540) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 44 kD, Observed band size: 47 kD.