LEF-1 (phospho Ser42) rabbit pAb

• Catalog Number: orb769376
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LEF-1 (phospho-Ser42)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human LEF-1 around the phosphorylation site of Ser42. AA range:8-57
• MW: 55
• Tested applications: ELISA, IF, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-LEF1 antibody, anti-Lymphoid enhancer-binding
• Note: For research use only

Western Blot analysis of 293 cells using Phospho-LEF-1 (S42) Polyclonal Antibody.

Western blot analysis of lysates from 293 cells treated with paclitaxel 1uM 24h, using LEF-1 (Phospho-Ser42) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).