Phospho-LEF1 (Ser42) Rabbit Polyclonal Antibody

SKU: orb1176

Description

Phospho-LEF1 (Ser42) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42 EI(p-S)HP
• Target: LEF1
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LEF1(phospho S42); LEF1(phospho Ser42); p-LEF1(phospho S42); p-LEF1(Ser42); lymphoid enhancing factor-1; Transcription factor T cell specific 1 alpha; DKFZp586H0919; LEF 1; LEF1; Lymphoid enhancer binding factor 1; T cell specific transcription factor 1 alpha; TCF 1 alpha; TCF1 alpha; TCF1alpha; TCF1-alpha; LEF1_HUMAN; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha.

Sample: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-LEF1 (Ser42) (orb1176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 58 kD.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Spleen tissue lysates, Primary: Anti-phospho-LEF1 (Ser42) (orb1176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 58 kDa.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-LEF1 (Ser42) Polyclonal Antibody, Unconjugated (orb1176) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

LEF1

UniProt

Q9UJU2