Phospho-LEF1 (Ser42) Rabbit Polyclonal Antibody

• Catalog Number: orb1176
• Category: Antibodies
• Description: Phospho-LEF1 (Ser42) Rabbit Polyclonal Antibody
• Target: LEF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42 EI(p-S)HP
• UniProt ID: Q9UJU2
• MW: 44 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LEF1(phospho S42); LEF1(phospho Ser42); p-LEF1(pho
• Note: For research use only

Sample: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-LEF1 (Ser42) (orb1176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 58 kD.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Spleen tissue lysates, Primary: Anti-phospho-LEF1 (Ser42) (orb1176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 58 kDa.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-LEF1 (Ser42) Polyclonal Antibody, Unconjugated (orb1176) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.