Pyruvate Carboxylase Antibody

SKU: orb19731

Description

Goat polyclonal to Pyruvate Carboxylase

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Dilution Range: ELISA: 1:16000, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Pyruvate Carboxylase
• Protein Sequence: KFKEVKKAYVEANQ
• Molecular Weight: 130
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PC antibody, anti pyruvate carboxylase antibody, anti PCB antibody

Western blot analysis of Mouse Liver lysate using Pyruvate Carboxylase antibody

Immunohistochemical staining of Human Liver using Pyruvate Carboxylase antibody

Quick Database Links

Gene Symbol

Pyruvate Carboxylase

NCBI Gene ID

5091