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Pyruvate Carboxylase Antibody

SKU: orb19731

Description

Goat polyclonal to Pyruvate Carboxylase

Images & Validation

Tested ApplicationsELISA, FC, IHC
Dilution RangeELISA: 1:16000, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetPyruvate Carboxylase
Protein SequenceKFKEVKKAYVEANQ
Molecular Weight130
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PC antibody, anti pyruvate carboxylase antibody, anti PCB antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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