PC Antibody (N-term)

SKU: orb1937421

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD53327
• Immunogen: This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC. Antigen Region: 53-82 aa.
• Target: PC (HGNC:8636)
• Molecular Weight: 129634 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC

Anti-PC Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 130 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PC Antibody (N-term) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the PC antibody detected the PC protein (arrow).

PC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PC (HGNC:8636)

UniProt

P11498

RefSeq (Protein):NP_071504.2, NP_001035806.1, NP_000911.2