PCB Rabbit Polyclonal Antibody

SKU: orb1816971

Description

PCB Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PCB (21-120/1178aa)
• Target: PC
• Molecular Weight: 127 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PYC_HUMAN; Pyruvate carboxylase, mitochondrial; EC:6.4.1.1; Pyruvic carboxylase(PCB); PCB; Pcx;

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PCB) Polyclonal Antibody, Unconjugated (orb1816971) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Primary: Anti-PCB (orb1816971) at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution, Predicted band size: 127 kDa, Observed band size: 127 kDa.

Sample: Lane 1: Mouse Liver tissue lysates, Primary: Anti-PCB (orb1816971) at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution, Predicted band size: 127 kDa, Observed band size: 127 kDa.

Quick Database Links

Gene Symbol

PC

UniProt

P11498