PCB rabbit pAb Antibody

SKU: orb769363

Description

Rabbit polyclonal antibody to PCB

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PC. AA range:357-406
• Molecular Weight: 120
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-PC antibody, anti-Pyruvate carboxylase antibody, anti- mitochondrial antibody, anti-Pyruvic carboxylase antibody, anti-PCB antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PC Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using PC Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using PC antibody.