CD18 Antibody / ITGB2 / LFA-1

• Catalog Number: orb749614
• Category: Antibodies
• Description: It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers, LFA-1, Mac-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54), ICAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and iC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This mAb blocks these cellular interactions.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 68-5A5
• Tested applications: FACS, Functional Studies, IF
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for the CD18 antibody.
• Dilution range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD18 antibody is available for research use only.
• UniProt ID: P05107
• Storage: Store the CD18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD18 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.