Integrin β2 (phospho Thr758) rabbit pAb

SKU: orb768826

Description

Rabbit polyclonal antibody to Integrin beta 2 (phospho-Thr758)

Images & Validation

• Tested Applications: ELISA, IF
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758. AA range:720-769
• Molecular Weight: 170
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-ITGB2 antibody, anti-CD18 antibody, anti-MFI7 antibody, anti-Integrin beta-2 antibody, anti-Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody, anti-95 subunit beta antibody, anti-Complement receptor C3 subunit beta antibody, anti-CD antigen CD18 antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD18/ITGB2 (Phospho-Thr758) Antibody.

Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).