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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 3
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 85kDa band observed in lysates of cell lines K562, MOLT4 and U937 (calculated MW of 84.8kDa according to NP_00202.2). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | ITGB2 / LFA-1 (aa86-99) |
| Protein Sequence | ETQEDHNGGQKQLS |
| Molecular Weight | 84.8 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti ITGB2 antibody, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody, anti CD18 antibody, anti LAD antibody, anti LCAMB antibody, anti LFA-1 antibody, anti MAC-1 antibody, anti MF17 antibody, anti MFI7 antibody, anti cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor) antibody, anti cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta antibody, anti complement receptor C3 beta-subunit antibody, anti complement receptor C3 subunit beta antibody, beta 2 antibody, anti leukocyte cell adhesion molecule CD18 antibody, anti leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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