Goat anti-ITGAM / CD11B Antibody

SKU: orb19554

Description

Goat polyclonal antibody to CD11b

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Dilution range: ELISA: 1:32000, IHC-P: 10 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 127kDa according to NP_000623.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ITGAM / CD11B
• Molecular Weight: 127
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti Antigen CD11b p170 antibody, anti CD11 antigen like family member B antibody, anti CD11 antigen-like family member B antibody, anti CD11b antibody, anti CD11b antigen antibody, anti CD11b/CD18 antibody, anti CD49d antibody, anti Cell surface glycoprotein MAC 1 alpha subunit antibody, anti Cell surface glycoprotein MAC-1 subunit alpha antibody, anti Cell Surface Glycoprotein MAC1 Alpha Subunit antibody, anti Complement component 3 receptor 3 subunit antibody, anti Complement Component Receptor 3 Alpha antibody, anti Complement receptor type 3, alpha subunit antibody, anti CR 3 alpha chain antibody, anti CR-3 alpha chain antibody, anti CR3 Alpha Chain antibody, anti CR3 antibody, anti CR3A antibody, anti F730045J24Rik antibody, anti Integrin Alpha M antibody, anti Integrin alpha M chain antibody, anti integrin alpha M complement component 3 receptor 3 subunit antibody, anti Integrin alpha-M antibody, anti Integrin beta 2 alpha subunit antibody, anti ITGAM antibody, anti Leukocyte adhesion receptor MO1 antibody, anti MAC 1 antibody, anti Mac-1a antibody, anti MAC1 antibody, anti Mac1, alpha subunit antibody, anti MAC1A antibody, anti Macrophage antigen alpha polypeptide antibody, anti Mo1, alpha subunit antibody, anti Neutrophil adherence receptor alpha M subunit antibody, anti Neutrophil adherence receptor antibody

orb19554 (2µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Immunofluorescence analysis of U937 cells using CD11b antibody.

Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

8 µg/mL staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Quick Database Links

Gene Symbol

ITGAM / CD11B

NCBI Gene ID

3684