Anti-Hu CD18 Purified

• Catalog Number: orb44305
• Category: Antibodies
• Description: Mouse Monoclonal to CD18.
• Clonality: Monoclonal
• Clone Number: MEM-48
• Tested applications: FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD18
• Entrez: 3689
• UniProt ID: P05107
• RRID: AB_10995654
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD18 antibody, anti-Integrin beta2 subunit an
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.
• Expiration Date: 12 months from date of receipt.

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Anti-Hu CD18 Purified (clone MEM-48) reactivity pattern in WB application.