CD18 Antibody

SKU: orb44305

Description

Mouse Monoclonal to CD18.

Images & Validation

• Tested Applications: FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-48
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Target: CD18
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Anti-Hu CD18 Purified (clone MEM-48) reactivity pattern in WB application.

Quick Database Links

Gene Symbol

CD18

UniProt

P05107

NCBI Gene ID

3689