Beta Catenin Antibody

SKU: orb385990

Description

Beta Catenin is a dual function protein, regulating the coordination of cell-cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. Beta catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. Beta-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]

Research Area

Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the Beta Catenin antibody to be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 5H10
• Immunogen: A C-terminal 100 amino acid segment from chicken beta-Catenin was used as the immunogen for the Beta Catenin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone 5H10). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

Western blot testing of human 1) A431 and 2) A549 cell lysate with Beta Catenin antibody (clone 5H10). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Beta Catenin Antibody (clone 5H10). Confirmation of Integrity and Purity of the Antibody.

Flow cytometry testing of PFA-fixed human HeLa cells with Beta Catenin antibody (clone 5H10); Red = isotype control, Blue = Beta Catenin antibody.

Quick Database Links

UniProt

P35222