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Catalog Number | orb385990 |
---|---|
Category | Antibodies |
Description | Beta Catenin is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. Beta catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. Beta-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 5H10 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Mouse IgG1, kappa |
Immunogen | A C-terminal 100 amino acid segment from chicken beta-Catenin was used as the immunogen for the Beta Catenin antibody. |
Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Beta Catenin antibody is available for research use only. |
UniProt ID | P35222 |
Storage | Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Beta Catenin antibody to be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human tonsil tissue using Beta Catenin antibody
Western blot analysis of human A431(lane 1) and A549(lane 2) cell lysate using Beta Catenin antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P | |
Gallus, Porcine, Rabbit |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC | |
Human, Mouse, Rat | |
Unconjugated |
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