Beta catenin Rabbit Polyclonal Antibody

• Catalog Number: orb10246
• Category: Antibodies
• Description: Beta catenin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep, Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Beta catenin (661-781/781aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 9.5/86 kDa
• Target: CTNNB1
• UniProt ID: P35222
• RRID: AB_10751994
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: beta-catenin; CHBCAT; CTNNB1; CTNNB; PRO2286; Cadh
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela (blue). Primary Antibody: Rabbit Anti-Beta catenin antibody (orb10246, Green), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature. Primary antibody (orb10246, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta catenin) Polyclonal Antibody, Unconjugated (orb10246) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Beta catenin (orb10246) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 9.5/86 kD, Observed band size: 86 kD.

Tissue/cell: rat skin tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Primary Antibody: Anti-Beta catenin Polyclonal Antibody, Unconjugated (orb10246) 1:200, overnight at 4°C, Secondary Antibody: IHC: Goat Anti-Rabbit IgG, Biotin conjugated + Streptavidin, HRP conjugated, and DAB staining, IF: Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C, Excitation wavelength: 565nm, Emission wavelength:578nm.