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Anti-beta Catenin/CTNNB1 Antibody

Catalog Number: orb402308

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb402308
CategoryAntibodies
DescriptionAnti-beta Catenin/CTNNB1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW95 kDa
UniProt IDP35222
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCatenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cellsbr> ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-beta Catenin/CTNNB1 Antibody

Flow Cytometry analysis of Hela cells using anti-CTNNB1 antibody. Overlay histogram showing Hela cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNB1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-beta Catenin/CTNNB1 Antibody

IF analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-CTNNB1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-beta Catenin/CTNNB1 Antibody

IF analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-CTNNB1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-beta Catenin/CTNNB1 Antibody

IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-beta Catenin/CTNNB1 Antibody

IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-beta Catenin/CTNNB1 Antibody

IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-beta Catenin/CTNNB1 Antibody

IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-beta Catenin/CTNNB1 Antibody

IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-beta Catenin/CTNNB1 Antibody

Western blot analysis of CTNNB1 using anti-CTNNB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.

Anti-beta Catenin/CTNNB1 Antibody

Western blot analysis of CTNNB1 using anti-CTNNB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.

  • Anti-beta Catenin CTNNB1 Antibody (monoclonal, 1F6) [orb421109]

    FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-beta Catenin/CTNNB1 Antibody [orb154595]

    ICC,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-beta Catenin/CTNNB1 Antibody [orb18119]

    ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-beta Catenin CTNNB1 Rabbit Monoclonal Antibody [orb546971]

    ICC,  IF,  IHC,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-beta Catenin/CTNNB1 Antibody [orb2579723]

    ICC,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg