Beta-catenin

• Catalog Number: orb1294385
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Beta Catenin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:300
• Conjugation: Unconjugated
• Target: Beta-catenin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Beta Catenin antibody at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294385 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.

Immunohistochemical analysis of paraffin embedded Human HER2+ Breast carcinoma tissue labeling Beta Catenin with orb1294385 at 1/200.

Immunohistochemical analysis of paraffin embedded Human HER2+ Breast carcinoma tissue labeling Beta Catenin with orb1294385 at 1/200.

Anti-Beta Catenin antibody at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294385 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294385 anti-Beta Catenin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Application: FFPE immunohistochemistry; Condition/Dilution 1-200; Epitope Retrieval methods: Citrate Buffer, pH 6.0; Sample: Rat skin.

Application: FFPE immunohistochemistry; Condition/Dilution 1-200; Epitope Retrieval methods: Citrate Buffer, pH 6.0; Sample: Rat skin.