Beta Catenin Antibody

• Catalog Number: orb385983
• Category: Antibodies
• Description: Beta-Catenin associates with the cytoplasmic portion of E-Cadherin, which is necessary for the function of E-Cadherin as an adhesion molecule. In normal tissues, beta-Catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-Catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immunostaining of beta-Catenin and E-Cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTNNB1/1507
• Tested applications: IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: A partial human recombinant protein was used as the immunogen for the Beta Catenin antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Beta Catenin antibody is available for research use only.
• UniProt ID: P35222
• Storage: Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Beta Catenin antibody to be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human tonsil tissue using Beta Catenin antibody

Immunohistochemical staining of Human pancreas tissue using Beta Catenin antibody

Immunohistochemical staining of Mouse colon tissue using Beta Catenin antibody

Immunohistochemical staining of Rat colon tissue using Beta Catenin antibody