Anti-Parkin Antibody

• Catalog Number: orb315645
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Parkin
• Target: PARK2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Parkin. The exact sequence is proprietary.
• UniProt ID: O60260
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PRKN antibody, anti E3 ubiquitin-protein liga
• Note: For research use only
• Entrez: 5071

Western blot analysis of Parkin expression in mouse kidney (A), mouse heart (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Parkin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Parkin staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).