Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody

• Catalog Number: orb312554
• Category: Antibodies
• Description: Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody
• Target: PRKN
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Monkey, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 QQ(p-S)IV
• UniProt ID: O60260
• MW: 52 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Parkin(phospho S65); p-Parkin(phospho S65); AR JP;
• Research Area: Cell Biology, Mitophagy Fission and Fusion
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chakrabarti, Lina et al. Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability MAbs, 14, 2020081 (2022)

PubMed: 35030984

Jamie Whitfield 1, Alannah K A McKay 2, Nicolin Tee 2, Rachel McCormick 2, Aimee Morabito 2, Leonidas G Karagounis 2 3 4, Andréane M Fouassier 5, Davide D'Amico 5, Anurag Singh 5, Louise M Burke 2, John A Hawley 2 6 Evaluating the Impact of Urolithin A Supplementation on Running Performance, Recovery, and Mitochondrial Biomarkers in Highly Trained Male Distance Runners Sports Medicine, (2025)

PubMed: 40839339

Applications

WB

Reactivity

Human

Huang, Yi-Fan et al. The protective effort of GPCR kinase 2-interacting protein-1 in neurons via promoting Beclin1-Parkin induced mitophagy at the early stage of spinal cord ischemia-reperfusion injury FASEB J, 34, 2055-2074 (2020)

PubMed: 31908016

Yu, Shao-Hua et al. Human antigen R regulates hypoxia-induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions J Cell Mol Med, 25, 2691-2702 (2021)

PubMed: 33496385

Lane, Sydney L. et al. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice Int J Mol Sci, 22, 13019 (2021)

PubMed: 34884824

Sun, Xin et al. Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy Acta Pharm Sin B, 12, 838-852 (2022)

PubMed: 35256949

Zhang, Qin et al. Regulation of mitophagy through HIF-1?/miR-140-5p/PARKIN axis in acute kidney injury Environ Toxicol, 37, 1759-1767 (2022)

PubMed: 35312153

Rai, Rajani et al. Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2 Front Oncol, 12, 958536 (2022)

PubMed: 36203464

Singh, Anurag et al. Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults Cell Rep Med, 3, 100633 (2022)

PubMed: 35584623

Chao, Tony et al. Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine Am. J. Physiol., Cell Physiol., (2019)

PubMed: 31532719

Sample: A673 (Human) cell Lysate at 40 ug, Primary: Anti-p-Parkin (Ser65) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (orb312554) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Parkin (Ser65) (orb312554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 50 kD.

Sample: Thymus (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti-phospho-Parkin (Ser65) (orb312554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD.