Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody

SKU: orb312554

Description

Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Monkey, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 QQ(p-S)IV
• Target: PRKN
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AR-JP; LPRS2; PARK2; PDJ; PRKN_HUMAN; PRKN; Parkin; Parkin RBR E3 ubiquitin-protein ligase; Parkinson juvenile disease protein 2 (Parkinson disease protein 2); 2.3.2.31; parkin RBR E3 ubiquitin protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson protein 2, E3 ubiquitin protein ligase (parkin); E3 ubiquitin ligase; PRKN | Parkin (phospho-S65); p-Parkin; phospho-Parkin; p-PRKN; PRKN | Parkin (phospho-Ser65)

Quick Database Links

Gene Symbol

PRKN

UniProt

O60260

Chakrabarti, Lina et al. Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability MAbs, 14, 2020081 (2022)

PubMed: 35030984

Jamie Whitfield 1, Alannah K A McKay 2, Nicolin Tee 2, Rachel McCormick 2, Aimee Morabito 2, Leonidas G Karagounis 2 3 4, Andréane M Fouassier 5, Davide D'Amico 5, Anurag Singh 5, Louise M Burke 2, John A Hawley 2 6 Evaluating the Impact of Urolithin A Supplementation on Running Performance, Recovery, and Mitochondrial Biomarkers in Highly Trained Male Distance Runners Sports Medicine, (2025)

PubMed: 40839339

Applications

WB

Reactivity

Human

Huang, Yi-Fan et al. The protective effort of GPCR kinase 2-interacting protein-1 in neurons via promoting Beclin1-Parkin induced mitophagy at the early stage of spinal cord ischemia-reperfusion injury FASEB J, 34, 2055-2074 (2020)

PubMed: 31908016

Yu, Shao-Hua et al. Human antigen R regulates hypoxia-induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions J Cell Mol Med, 25, 2691-2702 (2021)

PubMed: 33496385

Lane, Sydney L. et al. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice Int J Mol Sci, 22, 13019 (2021)

PubMed: 34884824

Sun, Xin et al. Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy Acta Pharm Sin B, 12, 838-852 (2022)

PubMed: 35256949

Zhang, Qin et al. Regulation of mitophagy through HIF-1?/miR-140-5p/PARKIN axis in acute kidney injury Environ Toxicol, 37, 1759-1767 (2022)

PubMed: 35312153

Rai, Rajani et al. Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2 Front Oncol, 12, 958536 (2022)

PubMed: 36203464

Singh, Anurag et al. Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults Cell Rep Med, 3, 100633 (2022)

PubMed: 35584623

Chao, Tony et al. Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine Am. J. Physiol., Cell Physiol., (2019)

PubMed: 31532719