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Phospho-PARK2 (Ser378) Rabbit Polyclonal Antibody

Catalog Number: orb6646

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6646
CategoryAntibodies
DescriptionPhospho-PARK2 (Ser378) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityGuinea pig, Porcine
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human PARK2 around the phosphorylation site of Ser378 EC(p-S)AV
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW51 kDa
TargetPRKN
UniProt IDO60260
RRIDAB_10955180
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesParkin(phospho S378); p-Parkin(phospho S378); PARK
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-PARK2 (Ser378) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PARK2 (Ser378)) Polyclonal Antibody, Unconjugated (orb6646) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PARK2 (Ser378) Rabbit Polyclonal Antibody

Sample: Kidney (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Primary: Anti-phospho-PARK2 (Ser378) (orb6646) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Phospho-PARK2 (Ser378) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-phospho-PARK2 (Ser378) (orb6646) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kDa, Observed band size: 47 kDa.