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Parkin Rabbit Polyclonal Antibody

Catalog Number: orb526783

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb526783
CategoryAntibodies
DescriptionParkin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Porcine, Rat, Sheep
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Parkin (261-465/465aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW51 kDa
TargetPRKN
UniProt IDO60260
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesParkin protein; AR JP; E3 ubiquitin protein ligase
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NoteFor research use only
Expiration Date12 months from date of receipt.
Parkin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Parkin) Polyclonal Antibody, Unconjugated (orb526783) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Parkin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Parkin) Polyclonal Antibody, Unconjugated (orb526783) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Parkin Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Mouse Hippocampus Lysates, Primary: Anti-Parkin (orb526783) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kDa, Observed band size: 51kDa.

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