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Catalog Number | orb234353 |
---|---|
Category | Antibodies |
Description | Anti-Parkin/PRKN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively. |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 66 kDa |
UniProt ID | O60260 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | E3 ubiquitin-protein ligase parkin; Parkin; 6.3.2. Read more... |
Note | For research use only |
Application notes | Immunocytochemistry , 0.5-1μg/ml, Mouse, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, RatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Anti-Parkin Picoband antibody, IHC(P): Human Intestinal Cancer Tissue.
Anti-Parkin Picoband antibody, IHC(P): Mouse Brain Tissue.
Anti-Parkin Picoband antibody, IHC(P): Rat Brain Tissue.
Anti-Parkin Picoband antibody, Western blotting. All lanes: Anti Parkin at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted bind size: 66 KD. Observed bind size: 66 KD.
IHC analysis of Parkin using anti-Parkin antibody. Parkin was detected in immunocytochemical section of NEURO-2α Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Parkin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |