Parkin/PRKN Rabbit Polyclonal Antibody

SKU: orb234353

Description

Parkin/PRKN Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Immunocytochemistry, 0.5-1μg/ml, Mouse, - Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
• Target: E3 ubiquitin-protein ligase parkin
• Molecular Weight: 66 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase parkin; Parkin; 6.3.2.-; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN

Anti-Parkin Picoband antibody, IHC(P): Human Intestinal Cancer Tissue.

Anti-Parkin Picoband antibody, IHC(P): Mouse Brain Tissue.

Anti-Parkin Picoband antibody, IHC(P): Rat Brain Tissue.

Anti-Parkin Picoband antibody, Western blotting. All lanes: Anti Parkin at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted bind size: 66 KD. Observed bind size: 66 KD.

IHC analysis of Parkin using anti-Parkin antibody. Parkin was detected in immunocytochemical section of NEURO-2α Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Parkin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

E3 ubiquitin-protein ligase parkin

UniProt

O60260