CD18 Antibody

SKU: orb154416

Description

Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1). CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, Functional Studies, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex. Western blotting: Recommended dilution: 1-2 μg/ml.Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-48
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Target: CD18
• Purification: Purified by sequential steps of physicochemical fractionation (caprylic acid precipitation and ammonium sulfate precipitation).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Quick Database Links

Gene Symbol

CD18

UniProt

P05107

NCBI Gene ID

3689