Anti-Hu CD18 Purified Low Endotoxin

• Catalog Number: orb154418
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1). CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression.
• Clonality: Monoclonal
• Clone Number: MEM-148
• Tested applications: FA, FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Peripheral blood mononuclear cells
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. Flow cytometry: Recommended dilution: 8 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD18
• Entrez: 3689
• UniProt ID: P05107
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-Integrin beta2 antibody, anti-Complement rece
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 μg/ml) GAM APC.