CD18 Antibody

SKU: orb154418

Description

Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1). CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression.

Images & Validation

• Tested Applications: FC, Functional Studies, IP, WB
• Reactivity: Human
• Application Notes:
  Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. Flow cytometry: Recommended dilution: 1-5 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-148
• Immunogen: Peripheral blood mononuclear cells
• Target: CD18
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD18

UniProt

P05107

NCBI Gene ID

3689