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Item 1 of 3
Anti-beta-Catenin Purified
Catalog Number: orb43778
| Catalog Number | orb43778 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to beta Catenin. |
| Clonality | Monoclonal |
| Clone Number | EM-22 |
| Tested applications | FC, ICC, IP, WB |
| Reactivity | Hamster, Human, Mouse |
| Isotype | Mouse IgG2a |
| Immunogen | Recombinant human beta-catenin |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | beta-Catenin |
| Entrez | 1499 |
| UniProt ID | P35222 |
| RRID | AB_10991585 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-beta-Catenin antibody Read more... |
| Note | For research use only |
| Application notes | Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line. Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.
Separation of MCF-7 cells stained using anti-beta-Catenin (EM-22) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue).
