beta-Catenin Antibody

• Catalog Number: orb43778
• Category: Antibodies
• Description: Mouse Monoclonal to beta Catenin.
• Target: beta-Catenin
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Hamster, Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Recombinant human beta-catenin
• UniProt ID: P35222
• RRID: AB_10991585
• Tested applications: FC, ICC, IP, WB
• Application notes: Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line. Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: EM-22
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTNNB1, EVR7, MRD19
• Research Area: Epigenetics, Infectious Diseases, Neuroscience, Si
• Note: For research use only
• Entrez: 1499
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.

Separation of MCF-7 cells stained using anti-beta-Catenin (EM-22) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue).