ATG5 Antibody [KO/KD Validated]

• Catalog Number: orb665772
• Category: Antibodies
• Description: The ATG5 Antibody [KO/KD Validated] is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATG5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATG5. The exact sequence is proprietary.
• UniProt ID: Q9H1Y0
• Tested applications: IHC, WB
• Dilution range: WB: 1-500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APG5L; ASP; Autophagy protein 5; APG5-like; Apopto
• Research Area: Apoptotic, Autophagic, Cancer, Epigenetics, Immuno
• Note: For research use only
• Entrez: 9474

Western blot analysis of ATG5 expression in HepG2 (A), Myla2059 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 55 kD)

Immunohistochemical analysis of ATG5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.