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ATG5/APG5L Rabbit Polyclonal Antibody

Catalog Number: orb5704

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5704
CategoryAntibodies
DescriptionATG5/APG5L Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human APG5L (101-200/275aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW32 kDa
TargetATG5
UniProt IDQ9H1Y0
RRIDAB_10926251
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like;
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NoteFor research use only
Expiration Date12 months from date of receipt.
ATG5/APG5L Rabbit Polyclonal Antibody

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ATG5 antibody (orb5704), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ATG5/APG5L Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ATG5/APG5L Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ATG5/APG5L Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ATG5/APG5L Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb5704) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

ATG5/APG5L Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-ATG5/APG5 (orb5704) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 47 kDa.

  • APG5 Antibody [orb1933443]

    IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • ATG5 Antibody (C-term) [orb1933444]

    IF,  IHC-P,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • ATG5 Antibody (N-term) [orb1933445]

    IF,  IHC-P,  WB

    Porcine, Rat, Zebrafish

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • ATG5/APG5L Rabbit Polyclonal Antibody [orb1152149]

    IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Gallus, Human, Porcine, Sheep

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
  • ATG5/APG5L Rabbit Polyclonal Antibody (HRP) [orb480165]

    IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl