ATG5 Antibody (C-term)

• Catalog Number: orb1933444
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9H1Y0
• MW: 32447 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Clone Number: RB7547
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Autophagy protein 5, APG5-like, Apoptosis-specific
• Note: For research use only
• NCBI: NP_004840.1

Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). Diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 μM, 16 h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Cos7, HEK293, MEF, and Hela cells left to right, respectively.

Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates.

Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.