ATG5 Antibody

SKU: orb411603

Description

The ATG5 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human ATG5 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human ATG5
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

Western blot analysis of ATG5 expression in Hela (A), NIH3T3 (B), PC12 (C), rat bain (D) whole cell lysates. (Predicted band size: 22; 32 kD; Observed band size: 55 kD)

Immunofluorescent analysis of ATG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q9H1Y0, Q3MQ06, Q99J83

NCBI Gene ID

9474, 11793