AIF1/IBA1 Antibody

• Catalog Number: orb1537846
• Category: Antibodies
• Description: AIF1/IBA1 Antibody
• Target: AIF1 / IBA1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Hamster, Human, Monkey, Mouse, Porcine, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence C-TGPPAKKAISELP, from the C-Terminus of protein sequence according to NP_116573.1NP_001614.3.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B2645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2645 was determined to be 5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1
• Note: For research use only

AIF1 / IBA1 antibody (1 µg/ml) staining of Human Brain (Frontal Cortex (A) Rat (B) Mouse (C) Brain and Swine E) Brain lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.